After the death of humans or animals, the odors released at different stages of decay attract various insects, and other arthropods, to the corpses. Therefore, the development of insects, and other arthropods present on corpses, can be assessed to estimate the minimum postmortem interval since death. In general, necrophagous blow flies are the insects that first colonize corpses. With progressing decay, other necrophagous and predatory insects arrive at the corpses, which will develop on or around these either by feeding directly on the corpses or by prey on other immature insects. Beetles (Coleoptera) mainly arrive at the corpses during the later stages of decay, and play important roles in cases with longer postmortem intervals. Necrobia rufipes (De Geer, 1755) (Coleoptera: Cleridae) is an important stored-product species with world-wide distribution. Moreover, it is also a forensically important insect species. At temperatures of 22, 25, 28, 31, 34, and 36°C (±0.5°C), the developmental periods from egg to adult were 113.20±2.96, 66.16±3.22, 50.61±1.95, 38.26±2.48, 37.97±2.40, and 31.20±2.11 days, respectively. In vivo measurements obtained the morphological indexes of larvae. The growth curve and the equation of the relationship between development time, body lengths, and mesonotum widths were simulated. The isomorphen diagram model, the isomegalen diagram model, and the thermal summation model were established. In addition, the widths of head capsules and pronota of larvae at different instars were determined by cluster analysis. Classifiers were created and validated by linear discriminant analysis.
Keywords: Development; Forensic entomology; In vivo measurement; Instar determination; Necrobia rufipes.
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