A 3D Brillouin microscopy dataset of the in-vivo zebrafish eye

Héctor Sánchez-Iranzo; Carlo Bevilacqua; Alba Diz-Muñoz; Robert Prevedel

doi:10.1016/j.dib.2020.105427

A 3D Brillouin microscopy dataset of the in-vivo zebrafish eye

Data Brief. 2020 Mar 17:30:105427. doi: 10.1016/j.dib.2020.105427. eCollection 2020 Jun.

Authors

Héctor Sánchez-Iranzo¹, Carlo Bevilacqua¹, Alba Diz-Muñoz¹, Robert Prevedel^{1

2

3

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Affiliations

¹ Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
² Developmental Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
³ Epigenetics and Neurobiology Unit, European Molecular Biology Laboratory, Monterotondo, Italy.
⁴ Molecular Medicine Partnership Unit, European Molecular Biology Laboratory, Heidelberg, Germany.

Abstract

In this work we present three-dimensional (3D) measurements of Brillouin scattering spectra of the in-vivo zebrafish larvae eye. This dataset was obtained by Brillouin microscopy, an emerging all-optical and non-contact technique that gives access to material properties through the process of Brillouin scattering. Herein, we share a representative 3D dataset of spectral properties of 48-52 h post-fertilization (hpf) zebrafish embryos. These spectral properties can be related to a complex longitudinal modulus and thus elastic and viscous properties given knowledge of refractive index and material density. The dataset encompasses the crystalline lens as well as several different retinal layers. This data provides a valuable resource as well as a starting point for researchers interested in the mechanobiology of vertebrate eye development.

Keywords: Brillouin microscopy; Brillouin scattering; Elasticity; Mechanobiology; Viscosity; Zebrafish larvae.