SLC30A8, CDKAL1, TCF7L2, KCNQ1 and IGF2BP2 are Associated with Type 2 Diabetes Mellitus in Iranian Patients

Kazem Vatankhah Yazdi; Seyed Mehdi Kalantar; Massoud Houshmand; Masoud Rahmanian; Masoud Reza Manaviat; Mohammad Reza Jahani; Behnam Kamalidehghan; Amir Almasi-Hashiani

doi:10.2147/DMSO.S225968

SLC30A8, CDKAL1, TCF7L2, KCNQ1 and IGF2BP2 are Associated with Type 2 Diabetes Mellitus in Iranian Patients

Diabetes Metab Syndr Obes. 2020 Mar 24:13:897-906. doi: 10.2147/DMSO.S225968. eCollection 2020.

Authors

Kazem Vatankhah Yazdi¹, Seyed Mehdi Kalantar¹, Massoud Houshmand^{2

3}, Masoud Rahmanian⁴, Masoud Reza Manaviat⁵, Mohammad Reza Jahani⁶, Behnam Kamalidehghan^{2

7}, Amir Almasi-Hashiani⁸

Affiliations

¹ Department of Genetic, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran.
² Department of Medical Genetics, National Institute for Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
³ Research Center, Knowledge University, Erbil, Kurdistan Region, Iraq.
⁴ Yazd Diabetes Research Center, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran.
⁵ Department of Ophthalmology, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran.
⁶ Taban Health Care and Diabetes Clinic Lab, Tehran, Iran.
⁷ Medical Genetics Department, Jiroft University of Medical Sciences and Health Services, Jiroft, Iran.
⁸ Department of Epidemiology, School of Health, Arak University of Medical Sciences, Arak, Iran.

Abstract

Background: Type 2 diabetes mellitus (T2DM) is a serious public health issue with significantly increasing rates across the world. The genome-wide association studies (GWAS) have previously manifested involved genes that remarkably enhance the risk of T2DM. In this study, the association of common variants with T2DM risk has been identified among Iranian population from Tehran province of Iran.

Methods: Here, the association of refSNPs with T2DM risk was examined on peripheral blood samples of 268 individuals including control group and patients with T2DM using the tetra amplification refractory mutation system (ARMS) methods and direct genomic DNA sequencing.

Results: Our study demonstrated that SLC30A8 rs13266634 (T/C), CDKAL1 rs10946398 (A/C), TCF7L2 rs7903146 (C/T), KCNQ1 rs2237892 (T/C), and IGF2BP2 rs1470579 (A/C) polymorphisms are significantly associated with type 2 diabetes, but no significant association was identified for FTO rs8050136 and MTNR1B rs10830963 polymorphisms.

Conclusion: The prediction of refSNPs is remarkably needed for pharmacogenetics and pharmacogenomic approaches, in which the information would be useful for clinicians to optimize therapeutic strategies and adverse drug reactions in patients with T2DM.

Keywords: ARMS; GWAS; Iranian populations; T2DM; the genome-wide association studies; the tetra amplification refractory mutation system; type 2 diabetes mellitus.