Whole-Genome Draft Assemblies of Difficult-to-Classify Escherichia coli O157 and Non-O157 Isolates from Feces of Canadian Feedlot Cattle

Vinicius Silva Castro; Eduardo Eustáquio de Souza Figueiredo; Tim McAllister; Robin King; Tim Reuter; Rodrigo Ortega Polo; Carlos Adam Conte Jr; Kim Stanford

doi:10.1128/MRA.00168-20

Whole-Genome Draft Assemblies of Difficult-to-Classify Escherichia coli O157 and Non-O157 Isolates from Feces of Canadian Feedlot Cattle

Microbiol Resour Announc. 2020 Apr 9;9(15):e00168-20. doi: 10.1128/MRA.00168-20.

Authors

Vinicius Silva Castro¹, Eduardo Eustáquio de Souza Figueiredo², Tim McAllister³, Robin King⁴, Tim Reuter⁵, Rodrigo Ortega Polo³, Carlos Adam Conte Jr¹, Kim Stanford⁶

Affiliations

¹ Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
² Department of Food and Nutrition, Federal University of Mato Grosso, Cuiaba, Brazil.
³ Agriculture and Agri-Food Canada, Lethbridge Research and Development Centre, Lethbridge, Canada.
⁴ Alberta Agriculture and Forestry, Edmonton, Canada.
⁵ Alberta Agriculture and Forestry, Agriculture Centre, Lethbridge, Canada.
⁶ Alberta Agriculture and Forestry, Agriculture Centre, Lethbridge, Canada Kim.Stanford@gov.ab.ca.

Abstract

Forty-eight Escherichia coli strains were chosen due to variable detection of stx or serogroup by PCR. Although all strains were initially determined to be Shiga toxin-producing Escherichia coli (STEC), their genomes revealed 11 isolates carrying stx _1a, stx _1b, stx _2a, and/or stx _2b Assembled genome sizes varied between 4,667,418 and 5,556,121 bp, with N ₅₀ values between 79,648 and 294,166 bp and G+C contents between 50.3% and 51.4%.