Abstract
A ratiometric fluorescent probe (PNA) was developed to sense and image endogenous bacterial penicillin G acylase (PGA). Oleanolic acid was discovered as a potential natural inhibitor of PGA using high-throughput screening techniques based on PNA.
MeSH terms
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Bacteria / enzymology*
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Fluorescent Dyes / pharmacology*
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High-Throughput Screening Assays
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Microscopy, Confocal
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Naphthalimides / pharmacology*
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Oleanolic Acid
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Optical Imaging
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Penicillin Amidase / antagonists & inhibitors*
Substances
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Fluorescent Dyes
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Naphthalimides
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Oleanolic Acid
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Penicillin Amidase