Visualization of penicillin G acylase in bacteria and high-throughput screening of natural inhibitors using a ratiometric fluorescent probe

Chem Commun (Camb). 2020 Apr 30;56(34):4640-4643. doi: 10.1039/d0cc00197j. Epub 2020 Apr 9.

Abstract

A ratiometric fluorescent probe (PNA) was developed to sense and image endogenous bacterial penicillin G acylase (PGA). Oleanolic acid was discovered as a potential natural inhibitor of PGA using high-throughput screening techniques based on PNA.

MeSH terms

  • Bacteria / enzymology*
  • Fluorescent Dyes / pharmacology*
  • High-Throughput Screening Assays
  • Microscopy, Confocal
  • Naphthalimides / pharmacology*
  • Oleanolic Acid
  • Optical Imaging
  • Penicillin Amidase / antagonists & inhibitors*

Substances

  • Fluorescent Dyes
  • Naphthalimides
  • Oleanolic Acid
  • Penicillin Amidase