Generation of a TLR7 heterozygous knockout line (WAe009-A-18) from human embryonic stem cells using CRISPR/Cas9

Hyang-Hee Seo; Hyo-Won Han; Jung-Hyun Kim

doi:10.1016/j.scr.2020.101735

Generation of a TLR7 heterozygous knockout line (WAe009-A-18) from human embryonic stem cells using CRISPR/Cas9

Stem Cell Res. 2020 May:45:101735. doi: 10.1016/j.scr.2020.101735. Epub 2020 Mar 13.

Authors

Hyang-Hee Seo¹, Hyo-Won Han¹, Jung-Hyun Kim²

Affiliations

¹ Division of Intractable Diseases, Center for Biomedical Sciences, Korea National Institute of Health, Cheongju 28159, Republic of Korea.
² Division of Intractable Diseases, Center for Biomedical Sciences, Korea National Institute of Health, Cheongju 28159, Republic of Korea. Electronic address: kjhcorea@korea.kr.

PMID: 32268246
DOI: 10.1016/j.scr.2020.101735

Abstract

Toll-like receptor 7 (TLR7) is a member of the toll-like receptor (TLR) family that is essential in the innate immune system. In this study, we established a heterozygous TLR7 knockout H9 cell line using CRISPR/Cas9. TLR7 knockout H9 cells maintained their pluripotency and exhibited the ability to differentiate into the three germ layers without any karyotype abnormalities.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CRISPR-Cas Systems / genetics
Cell Line
Heterozygote
Human Embryonic Stem Cells*
Humans
Toll-Like Receptor 7 / genetics

Substances

TLR7 protein, human
Toll-Like Receptor 7