Biocompatibility of biphasic α,β-tricalcium phosphate ceramics in vitro

T V Safronova; I I Selezneva; S A Tikhonova; A S Kiselev; G A Davydova; T B Shatalova; D S Larionov; J V Rau

doi:10.1016/j.bioactmat.2020.03.007

Biocompatibility of biphasic α,β-tricalcium phosphate ceramics in vitro

Bioact Mater. 2020 Apr 1;5(2):423-427. doi: 10.1016/j.bioactmat.2020.03.007. eCollection 2020 Jun.

Authors

T V Safronova¹, I I Selezneva², S A Tikhonova¹, A S Kiselev¹, G A Davydova², T B Shatalova¹, D S Larionov¹, J V Rau³

Affiliations

¹ Lomonosov Moscow State University, Leninskie Gory, 1, Moscow, 119991, Russia.
² Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, 142292, Moscow Region, Russia.
³ Istituto di Struttura Della Materia, Consiglio Nazionale Delle Ricerche (ISM-CNR), Via Del Fosso Del Cavaliere 100, 00133, Rome, Italy.

Abstract

The biocompatibility of biphasic α,β-tricalcium phosphate ceramics, obtained by annealing a compact preform based on β-tricalcium phosphate powder, was studied in vitro. It was found that within 10-30 days the adhesion of primary dental pulp stem cells located on the surface of biphasic α,β-tricalcium phosphate ceramics is suppressed. Decrease of the cell number on the surface of biphasic α,β-tricalcium phosphate ceramics, most likely, can be associated with both the pH level (acidic) as a result of hydrolysis of the more soluble phase of α-tricalcium phosphate and with the nature of surface that changes as a result of the formation and growth of hydroxyapatite crystals.

Keywords: Biocompatibility; Ceramics; Dental pulp stem cells; Phase composition; Tricalcium phosphate.