Additive anti-inflammation by a combination of conjugated linoleic acid and α-lipoic acid through molecular interaction between both compounds

Ki-June Lee; Yoon-Joo Ko; Sang-Kee Kang; Whee-Soo Kim; Chong-Su Cho; Yun-Jaie Choi

doi:10.1007/s10068-019-00677-7

Additive anti-inflammation by a combination of conjugated linoleic acid and α-lipoic acid through molecular interaction between both compounds

Food Sci Biotechnol. 2019 Oct 15;29(3):419-429. doi: 10.1007/s10068-019-00677-7. eCollection 2020 Mar.

Authors

Ki-June Lee¹, Yoon-Joo Ko², Sang-Kee Kang³, Whee-Soo Kim¹, Chong-Su Cho¹, Yun-Jaie Choi¹

Affiliations

¹ 1Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 08826 Republic of Korea.
² 2National Center for Inter-University Research Facilities, Seoul National University, Seoul, 08826 Republic of Korea.
³ 3Institute of Green-Bio Science and Technology, Seoul National University, Pyeongchang, Gangwon-do 25354 Republic of Korea.

Abstract

Alpha lipoic acid (LA) and conjugated linoleic acid (CLA) have been well-documented on a variety of functional effects in health foods. The main purpose of this study was focused on the additive anti-inflammatory activity of the combination of LA and CLA in vitro. Raw 264.7 cells induced by lipopolysaccharide were treated with LA and CLA individually or in combination at a variety of concentration ranges. Co-treating 25 μM of LA and 25 μM of CLA significantly inhibited pro-inflammatory cytokines compared to the same concentration of single LA- or CLA-treated group. The molecular mechanism of anti-inflammation by a combination of these compounds was attributed to extracellular signal-regulated kinase-1 (ERK1) and peroxisome proliferator-activated receptor gamma (PPARγ). Also, the molecular interaction between both compounds was confirmed by NMR. Our findings suggested that the combination of CLA and LA showed potential additive effect on anti-inflammation through the molecular interaction of both compounds.

Keywords: Alpha lipoic acid (LA); Anti-inflammation; Conjugated linoleic acid (CLA); Extracellular signal-regulated kinase-1 (ERK1); Peroxisome proliferator-activated receptor gamma (PPARγ).