Epigenetic Reprogramming During Somatic Cell Nuclear Transfer: Recent Progress and Future Directions

Xiangyu Wang; Jiadan Qu; Jie Li; Hongbin He; Zhonghua Liu; Yanjun Huan

doi:10.3389/fgene.2020.00205

Epigenetic Reprogramming During Somatic Cell Nuclear Transfer: Recent Progress and Future Directions

Front Genet. 2020 Mar 18:11:205. doi: 10.3389/fgene.2020.00205. eCollection 2020.

Authors

Xiangyu Wang¹, Jiadan Qu¹, Jie Li², Hongbin He³, Zhonghua Liu⁴, Yanjun Huan¹

Affiliations

¹ College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, China.
² Department of Cadre Health Care, Qingdao Municipal Hospital, Qingdao, China.
³ College of Life Sciences, Shandong Normal University, Jinan, China.
⁴ College of Life Sciences, Northeast Agricultural University, Harbin, China.

Abstract

Somatic cell nuclear transfer (SCNT) has broad applications but is limited by low cloning efficiency. In this review, we mainly focus on SCNT-mediated epigenetic reprogramming in livestock and also describe mice data for reference. This review presents the factors contributing to low cloning efficiency, demonstrates that incomplete epigenetic reprogramming leads to the low developmental potential of cloned embryos, and further describes the regulation of epigenetic reprogramming by long non-coding RNAs, which is a new research perspective in the field of SCNT-mediated epigenetic reprogramming. In conclusion, this review provides new insights into the epigenetic regulatory mechanism during SCNT-mediated nuclear reprogramming, which could have great implications for improving cloning efficiency.

Keywords: cloning efficiency; epigenetic modification; long non-coding RNA; nuclear reprogramming; somatic cell nuclear transfer.

Publication types

Review