Monitoring the hemostasis process through the electrical characteristics of a graphene-based field-effect transistor

Abstract

Graphene-based transistors are promising devices in the evaluation of carrier density in biological analytes. We report on the design and fabrication of a graphene-based field-effect transistor for monitoring and assessing the interaction between the coagulation factors based on the charge carrier density in a blood sample. When biochemical reactions occurred during the coagulation cascade process, a dopant effect was noticed on the graphene surface by the change in Dirac point voltage values. Additional experiments were performed using blood samples treated with activators (vitamin K, calcium chloride, and thromboplastin reagent) and inhibitors (heparin drugs) to evaluate the selectivity of the graphene field-effect transistor devices. Since the transfer characteristic curves presented divergent behaviours for different levels of procoagulants and anticoagulants, the measurements showed that the devices can assess changes in the concentrations of factors that inhibit or accelerate the cascade process when using untreated and treated samples. Reproducibility was verified by testing samples from different sources. To the best of our knowledge, this study is the first to demonstrate the potential of graphene in monitoring the hemostasis process through the analysis of the electrical properties of human whole blood.

Keywords: Biosensor; Field-effect transistor; Graphene; Hemostasis.