Terpenoids from the Chinese liverwort Odontoschisma grosseverrucosum and their antifungal virulence activity

Abstract

Eight undescribed terpenoids, namely, odongrossins A-H, together with two known terpenoids were isolated from Odontoschisma grosseverrucosum Stephani (Cephaloziaceae). Their structures were established based on NMR data, electronic circular dichroism (ECD) calculations, and single-crystal X-ray diffraction measurements. Odongrossin A and odongrossin G displayed moderate anti-virulence activities against CDR1-and CDR2-efflux-pump-deficient Candida albicans DSY654. Further investigation of odongrossin A revealed that it inhibited adhesion and biofilm formation on C. albicans DSY654. The results regarding the transcription levels of genes demonstrated that odongrossin A could regulate the expression of genes that are associated with the virulence of C. albicans DSY654.

Keywords: Anti-virulence; Candida albicans; Liverworts; Odongrossin A−H; Odontoschisma grosseverrucosum Stephani (cephaloziaceae); Terpenoids.