Immune-stimulating Effect of Lactobacillus plantarum Ln1 Isolated from the Traditional Korean Fermented Food, Kimchi

J Microbiol Biotechnol. 2020 Jun 28;30(6):926-929. doi: 10.4014/jmb.2001.01038.

Abstract

This study aimed to determine the immune-stimulating effects of heat-killed Lactobacillus plantarum Ln1 (HK-Ln1) through the production of nitric oxide (NO) and pro-inflammatory cytokine achieved by inducing NF-κB and mitogen-activated protein kinase (MAPK)-signaling pathways in macrophages. HK-Ln1 showed higher NO and cytokine production compared t°Control (nonstimulated lipopolysaccharide); in addition, the expression of inducible nitric oxide synthase (iNOS) was induced through HK-Ln1treatment. The phosphorylation of IκB-α and p65 increased following treatment by HK-Ln1, which implicates IκB-α degradation and the translocation of p65 to nucleus. In addition, the phosphorylation of MAPKs, ERK 1/2, JNK, and p38 was induced following HK-Ln1 treatment.

Keywords: Lactobacillus plantarum; heat-killed cells; immune-stimulating; probiotics.

MeSH terms

  • Animals
  • Cytokines / metabolism
  • Fermented Foods / microbiology*
  • Lactobacillus plantarum / immunology*
  • MAP Kinase Signaling System / drug effects
  • MAP Kinase Signaling System / immunology
  • Macrophages / immunology
  • Mice
  • Mitogen-Activated Protein Kinases / immunology
  • Mitogen-Activated Protein Kinases / metabolism
  • NF-kappa B / immunology
  • NF-kappa B / metabolism
  • Nitric Oxide / metabolism
  • Phosphorylation / immunology
  • Probiotics / pharmacology*
  • RAW 264.7 Cells
  • Republic of Korea

Substances

  • Cytokines
  • NF-kappa B
  • Nitric Oxide
  • Mitogen-Activated Protein Kinases