Objective: This preclinical research was designed to study the phosphorylation level of adducin in cancer tissues, healthy tissues, and malignant tumor cells to determine the relationship between adducin and cancer.
Methods: Western blotting was used to detect the expression level of phospho-adducin in tissues and cell lines.
Results: Phospho-adducin at Ser662 was detected in all tumor cells and cancer tissues. The main type of phospho-adducin at Ser662 was γ-adducin in healthy lung tissue, and α-adducin in both lung cancer tissue and para-lung cancer tissue. Phosphorylation of adducin at Thr445 was observed in healthy lung tissue, adjacent healthy tissue, and cancer tissue, but was not detected in any other malignant cells. Additionally, more phosphorylation of adducin at Thr445 was seen in cancer tissue than in adjacent healthy tissue.
Conclusion: The abnormal expression of phospho-adducin at Ser662 and Thr445 may be associated with tumorigenesis, suggesting a novel approach for the diagnosis and treatment of tumors.
Keywords: Lung cancer; Ser662; Thr445; adducin; carcinogenesis; phosphorylation.