Synthesis and biological characterization of Mn0.5Zn0.5EuxDyxFe1.8-2xO4 nanoparticles by sonochemical approach

Abstract

Metallic nanoparticles (NPs) possess unique properties which makes them attractive candidates for various applications especially in field of experimental medicine and drug delivery. Many approaches were developed to synthesize divers and customized metallic NPs that can be useful in many areas such as, experimental medicine, drug design, drug delivery, electrical and electronic engineering, electrochemical sensors, and biochemical sensors. Among different metallic nanoparticles, manganese (Mn) NPs are the most prominent materials, in the present study, we have synthetized unique Mn_0.5Zn_0.5Dy_xEu_xFe_1.8-2xO₄ NPs by using ultrasonication method (x ≤ 0.1). The structure, and surface morphology of Mn_0.5Zn_0.5Dy_xEu_xFe_1.8-2xO₄ NPs was characterized by XRD, SEM, TEM and EDX methods. We have examined the biological effects of Mn_0.5Zn_0.5Dy_xEu_xFe_1.8-2xO₄ NPs on both normal (HEK-293) and cancerous (HCT-116) cells. We have found that the treatment of Mn_0.5Zn_0.5Dy_xEu_xFe_1.8-2xO₄ NPs post 48 h, showed significant decline in cancer cells population as revealed by MTT assay. The IC₅₀ value of Mn_0.5Zn_0.5Dy_xEu_xFe_1.8-2xO₄ NPs was ranged between (2.35 μg/mL to 2.33 μg/mL). To check the specificity of the actions, we found that the treatment of Mn_0.5Zn_0.5Dy_xEu_xFe_1.8-2xO₄ NPs did not produce any effects on the normal cells, which suggest that Mn_0.5Zn_0.5Dy_xEu_xFe_1.8-2xO₄ NPs selectively targeted the cancerous cells. The anti-bacterial properties of Mn_0.5Zn_0.5Dy_xEu_xFe_1.8-2xO₄ NPs were also evaluated by MIC and MBC assays. We suggest that Mn_0.5Zn_0.5Dy_xEu_xFe_1.8-2xO₄ NPs produced by sonochemical method possess potential anti-cancer and anti-bacterial capabilities.

Keywords: Antibacterial activity; Anticancer activity; Biological characterization; Magnetic nanoparticles; Sonochemical synthesis.