Infecting Triatomines with Trypanosomes

Methods Mol Biol. 2020:2116:69-79. doi: 10.1007/978-1-0716-0294-2_5.

Abstract

The infection of triatomines with trypanosomes can be performed with different forms of the parasite, and the procedure is important not only for vector-parasite interaction studies but also for maintaining the infectivity of parasite strains, which guarantees more realistic biological and molecular investigations. Here, I describe how to infect the vector Rhodnius prolixus, a model species, with two different species of Trypanosoma.

Keywords: Artificial feeding; Infection; Rhodnius prolixus; Trypanosoma cruzi; Trypanosoma rangeli.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animal Feed
  • Animals
  • Disease Models, Animal
  • Host-Parasite Interactions
  • Humans
  • Insect Vectors / parasitology
  • Life Cycle Stages
  • Mice
  • Models, Animal
  • Parasitology / methods*
  • Rhodnius / parasitology*
  • Trypanosoma cruzi / isolation & purification
  • Trypanosoma cruzi / pathogenicity*
  • Trypanosoma cruzi / physiology
  • Trypanosoma rangeli / isolation & purification
  • Trypanosoma rangeli / pathogenicity*
  • Trypanosoma rangeli / physiology
  • Trypanosomiasis / parasitology
  • Trypanosomiasis / transmission*