Ziziphi Spinosae Folium, the leaf of Ziziphus jujuba Mill. Var. spinosa (Bunge) Hu ex H. F. Chou (LZJS), is currently used as a healthy tea in China. This study evaluated the chemical components and antioxidant activities of LZJS flavonoid (LZJSF) and fermented LZJSF (FLZJSF) using human intestinal bacteria (HIB) through dynamic fermentation. Eighteen flavonoids were simultaneously identified in LZJSF using UHPLC-Q-Orbitrap-MS method, nine of which were targeted for a HIB metabolism study. Seven small phenolic acids were identified in FLZJSF. Not only at chemical level but also at PC12 cell level, FLZJSF samples fermented for 4 and 6 h showed significant positive correlation between their activities and flavonoid aglycones, which were transformed from LZJSF. However, FLZJSF samples (8 h and longer time) mainly contained phenolic acids and indicated weak activities. Thus, LZJSF was found to result in increased antioxidant activity and could be commercially utilized as a novel functional food.
Keywords: 3, 4-Dihydroxyphenylacetic acid (Compound CID 547); Antioxidant; Flavonoids; Human intestinal bacteria; Kaempferol (Compound CID 5280863); Kaempferol-3-O-rutinoside (Compound CID 5318767); Quercetin (Compound CID 5280343); Quercetin-3-O-robinobioside (Compound CID 10371536); Quercetin-3-O-rutinoside (Compound CID 5280805); Quercetin-3-O-β-d-galactose (Compound CID 5281643); Quercetin-3-O-β-d-glucoside (Compound CID 5280804); Quercetin-3-rhamnoside (Compound CID 5280459); Taxifolin (Compound CID 439533); UHPLC-Q-Orbitrap-MS; Ziziphi Spinosae Folium.
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