Copper nanoclusters @ nitrogen-doped carbon quantum dots-based ratiometric fluorescence probe for lead (II) ions detection in porphyra

Abstract

A novel ratiometric fluorescence probe was proposed for detecting lead (II) ions (Pb²⁺) in porphyra, the approach was based on copper nanoclusters and nitrogen-doped carbon quantum dots (CuNCs-CNQDs). In this probe, the CuNCs delivered the response signal, the fluorescence of which was enhanced by Pb²⁺ due to the aggregation-induced emission enhancement (AIEE) between Pb²⁺ and CuNCs. The CNQDs provided the self-calibration signal, whose fluorescence remained almost unchanged in coexistence with Pb²⁺. According to the change of fluorescence intensity ratio between the fluorophores, CuNCs-CNQDs nanohybrid was used as ratiometric probes for the sensitive detection of Pb²⁺ in the range of 0.010-2.5 mg L^-1, with a detection limit of 0.0031 mg L^-1. Finally, the probe was successfully applied to detect Pb²⁺ in porphyra with relative standard deviations (RSDs) lower than 5%. This study provides a straightforward, stable, and sensitive approach for detecting Pb²⁺ in porphyra.

Keywords: Aggregation-induced emission enhancement; Copper nanoclusters; Lead (II) ions; Nitrogen-doped carbon quantum dots; Ratiometric fluorescence probe.