Isolation and molecular-based identification of bacteria from unhatched leftover eggs of ducks in selected mini-hatcheries of Kishoreganj, Bangladesh

Sadia Afrin Punom; Md Shahidur Rahman Khan; Shayka Tasnim Pritha; Jayedul Hassan; Saifur Rahman; Md Muket Mahmud; Md Shafiqul Islam

doi:10.5455/javar.2020.g406

Isolation and molecular-based identification of bacteria from unhatched leftover eggs of ducks in selected mini-hatcheries of Kishoreganj, Bangladesh

J Adv Vet Anim Res. 2020 Feb 19;7(1):164-169. doi: 10.5455/javar.2020.g406. eCollection 2020 Mar.

Authors

Sadia Afrin Punom¹, Md Shahidur Rahman Khan¹, Shayka Tasnim Pritha¹, Jayedul Hassan¹, Saifur Rahman¹, Md Muket Mahmud¹, Md Shafiqul Islam¹

Affiliation

¹ Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.

Abstract

Objectives: The study was designed for isolation and identification of the bacteria present in unhatched leftover eggs of duck in selected mini-hatcheries of Kishoreganj, Bangladesh.

Materials and methods: A total of 54 unhatched discarded eggs were collected as samples from different mini-hatcheries of Tarail and Itna Upazilas of Kishoreganj and aseptically carried to the laboratory in the icebox. Surface washings (n = 54) and inner contents (n = 54) were collected and enriched in Luria-Bertani broth followed by the isolation of pure colonies of different bacteria onto eosin methylene blue agar, mannitol salt agar, Salmonella-Shigella agar, and blood agar plates. Identification of the bacterial isolates was done by cultural properties, staining, and biochemical tests followed by molecular detection by Polymerase chain reaction.

Results: Of 108 samples, 62 were found positive for Salmonella spp. (76%), 59 for E. coli (54%), 52 for Staphylococcus spp. (48%), and 5 for Clostridium spp. (9%). From the egg surface samples, Staphylococcus spp. were recovered in the highest (67%) followed by Salmonella spp. (59%), E. coli (56%), and Clostridium spp. (9%). From the inner contents of eggs, Salmonella spp. were recovered in the highest (56%), followed by E. coli (53%) and Staphylococcus spp. (30%).

Conclusion: The isolated bacteria might be associated with the decreased hatchability and embryo mortality in the mini-hatcheries of duck.

Keywords: Bacteria; PCR; duck mini-hatchery; unhatched leftover eggs.