Conditioning adhesive contacts between the neutrophils and the endotheliocytes by Staphylococcus aureus

Abstract

We have developed a model for evaluating the integral intercellular interactions in the "endotheliocyte-neutrophil" system and have shown the high variability of adhesion contacts in different donors associated with different expression profiles of neutrophils. Two methods (forсe spectroscopy-spectroscopy and scanning ion-conductance microscopy) showed a decrease in the rigidity of the membrane-cytoskeletal complex of neutrophils under the influence of Staphylococcus aureus 2879 M. Adding this strain to the "endotheliocyte-neutrophil" system caused a statistically significant decrease in the adhesion force and adhesion work, which indicates a change in the expression profile and physicochemical properties of membranes of both types of interacting cells (neutrophils and endotheliocytes).

Keywords: Staphylococcus aureus; FS-spectroscopy; adhesion force; adhesion work; atomic-force microscopy; endotheliocyte; expression profile; intercellular contacts; neutrophil; scanning ion-conducting microscopy.