[Effect of moxibustion on body weight and peripheral and cerebral cortical IL-6 and IL-10 levels in fatigue rats]

Ya Zhao; Tian Ge Li; Rong Pu; Ling Shui; Jun Lu; Ying Song Chen

doi:10.13702/j.1000-0607.190815

[Effect of moxibustion on body weight and peripheral and cerebral cortical IL-6 and IL-10 levels in fatigue rats]

Zhen Ci Yan Jiu. 2020 Mar 25;45(3):215-9. doi: 10.13702/j.1000-0607.190815.

[Article in Chinese]

Authors

Ya Zhao¹, Tian Ge Li¹, Rong Pu², Ling Shui³, Jun Lu¹, Ying Song Chen³

Affiliations

¹ School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China.
² Eighth Medical Center, General Hospital of Chinese People's Liberation Army, Beijing 100091.
³ Mongolian Medical College, Inner Mongolia Medical University, Huhhot 010110.

PMID: 32202713
DOI: 10.13702/j.1000-0607.190815

Abstract

Objective: To observe the effect of moxibustion at "Zusanli"(ST36) on peripheral and central IL-6, IL-10 and IL-10/IL-6 changes in fatigue rats, so as to explore its mechanisms underlying relief of fatigue.

Methods: Twenty-one male SD rats were randomly divided into normal control, model and moxibustion groups with 7 rats in each group. The fatigue model was induced by exhausted weight-loaded swimming, once daily for 21 days. Moxibustion was applied to bilateral ST36 once every other day, for successive 11 times. The overall state and the body weight were observed. The levels of IL-6 and IL-10 in the serum, gastrocnemius and frontal cortex tissues were detected by enzyme-linked immunosorbent assay (ELISA).

Results: Compared with the normal group, the body weight was significantly reduced on the 7^th, 14^th, and 21^st day in the model group (P<0.05), and considerably increased on the 14^th and 21^st day in the moxibustion group relevant to the model group (P<0.05). After modeling, the levels of IL-6 in the serum, gastrocnemius and frontal cortex were significantly increased (P<0.05, P<0.01), while those of IL-10 was obviously decreased (P<0.05, P<0.01) in the model group relevant to the normal group, and ratio of IL-10/IL-6 was obviously decreased in the serum and frontal cortex of the model group relevant to the normal group (P<0.01). After the moxibustion intervention, the levels of IL-10 in the serum and frontal cortex were significantly increased (P<0.05), and ratio of IL-10/IL-6 in the serum and frontal cortex was significantly increased (P<0.01), while the IL-6 contents in the gastrocnemius and frontal cortex were notably decreased in the moxibustion group relevant to the model group (P<0.05).

Conclusion: Moxibustion can inhibit weight-loaded swimming induced decrease of body weight in fatigue rats, which may be associated with its effects in balancing the levels of peripheral and central pro-inflammatory cytokine IL-6 and anti-inflammatory cytokine IL-10.

Keywords: Body weight; Fatigue; Frontal cortex; Gastrocnemius; IL10a; IL6; Moxibustion; Serum.

MeSH terms

Acupuncture Points
Animals
Body Weight
Fatigue
Interleukin-10
Interleukin-6
Male
Moxibustion*
Rats
Rats, Sprague-Dawley

Substances

IL10 protein, human
Il6 protein, rat
Interleukin-6
Interleukin-10