In an extensive monitoring programme, event mean concentrations of 12 heavy metals, 16 polycyclic aromatic hydrocarbons (PAH), nine pesticides/biocides, three pharmaceuticals, three benzotriazoles, acesulfame, and DEHP (di-(2-ethylhexyl)phthalate) were measured at 10 combined sewer overflow (CSO) facilities throughout Bavaria, Germany, for more than 110 overflow events. A harmonised approach with large volume samplers was used to produce volume-proportional event composite samples. A wide range of event durations and volumes was covered successfully. All substances analysed were detected in CSO samples and the majority were quantified in more than 80% of the samples. Our results confirm that CSOs need to be considered in the debate on micropollutant emissions, and knowledge regarding their concentrations at a regional level needs to be solidified. Distinct substance-specific patterns can be observed in the variability between events and sites as well as in a correlation analysis of substance concentrations. These trends underline the need for differentiation of the substances by their predominant sources, pathways, and transport behaviours. Compared to wastewater treatment plants, CSOs are an important pollution source especially for ubiquitous, primarily stormwater-transported pollutants, including substances causing failure to achieve good chemical status of surface waters, such as the uPBT (ubiquitous, persistent, bioaccumulative and toxic) substances Hg and PAH.