Flavonoid pattern inheritance in the allopolyploid Spartina anglica - Comparison with the parental species S. maritima and S. alterniflora

Abstract

The invasive species Spartina anglica arose in Europe by a cross between the Afro-European species S. maritima (native, paternal ancestor) and the introduced North American S. alterniflora (invasive, maternal ancestor). Aqueous methanolic extracts were prepared from plant tissue for chemotaxonomical comparison between the three species and determination of the phenolic pattern inheritance in S. anglica. A total of 20 phenolic compounds were detected in the aerial tissues of S. anglica and S. alterniflora, but only seven in S. maritima. They were isolated from their respective crude extracts, and their structures were determined according to spectroscopic data analysis and chemical evidence. They all belong to the flavonoid class, with 13 of them identified as C-glycoflavonoid and seven as O-glycoflavonoid. All these products were detected for the first time from S. anglica, fourteen of them for the first time from S. alterniflora, and three of them for the first time from S. maritima. The individual concentrations in the three species were determined by quantitative HPLC. The two parental species were found to differ markedly in their foliar phenolic fingerprint, whereas that of S. anglica showed a clear maternal dominance. Eight of the fourteen major compounds identified were of maternal origin among which, six were over-expressed, only three were from paternal origin but under-regulated, while two originated from the two parents. As far as we know, this work represents the first exhaustive report of the phenolic fingerprints of S. alterniflora and S. anglica and of the phenolic pattern inheritance in S. anglica. The similarity in the phenolic chemistry of the introduced and invasive S. alterniflora to its progeny could play a role in the physiological vigour and invasion success of S. anglica. This work provide a foundation for further studies, considering the reported biological activities of C-glycosidic flavonoids and tricin derivatives, and the lack of knowledge of the ecological chemistry of the genus Spartina.

Keywords: (CID 101193641); (CID 114776); (CID 161672); (CID 25202838); (CID 3084995); (CID 442611); (CID 442658); (CID 5281702); (CID 72193659); (CID 9986192); (RN 1637757-97-1); (RN 220948-75-4); (RN 220948-77-6); (RN 32769-01-0); (RN 369390-51-2); (RN 369390-52-3); (RN 372113-55-8); (RN 380468-519); (RN 462100-42-2); Isoorientin; Isoorientin 2″-O-(6-(E)-p- caffeoylglucoside); Isoorientin 2″-O-(6-(E)-p-feruloylglucoside); Isoorientin 2″-O-(β-glucosyl); Isoschaftoside; Isoscoparin; Isoscoparin 2″-O-(6-(E)-p-feruloylglucoside); Isoscoparin 2″-O-(β-glucosyl); Isovitexin; Isovitexin 2″-O-(6-(E)-p-feruloylglucoside); Isovitexin 2″-O-(β-glucosyl); LC/MS; NMR; Quantitative HPLC; S. alterniflora; S. maritima; Schaftoside; Spartina anglica; Tricin; Tricin 4′-O-(erythro-β-guaiacylglyceryl) ether 7-O-β -glucoside; Tricin 4′-O-(threo-β-guaiacylglyceryl) ether 7-O-β-glucoside; Tricin 4′-O-Guaiacylglyceryl ether erythro; Tricin 4′-O-Guaiacylglyceryl ether threo; Tricin 7-O-Glucoside; Tricin 7-diglucuronide; flavonoid inheritance.