Dietary Supplementation With Bacillus subtilis Direct-Fed Microbials Alters Chicken Intestinal Metabolite Levels

Inkyung Park; Noah P Zimmerman; Alexandra H Smith; Thomas G Rehberger; Erik P Lillehoj; Hyun S Lillehoj

doi:10.3389/fvets.2020.00123

Dietary Supplementation With Bacillus subtilis Direct-Fed Microbials Alters Chicken Intestinal Metabolite Levels

Front Vet Sci. 2020 Mar 4:7:123. doi: 10.3389/fvets.2020.00123. eCollection 2020.

Authors

Inkyung Park¹, Noah P Zimmerman², Alexandra H Smith², Thomas G Rehberger², Erik P Lillehoj³, Hyun S Lillehoj¹

Affiliations

¹ Animal Bioscience and Biotechnology Laboratory, United States Department of Agriculture, Beltsville Agricultural Research Center, Agricultural Research Service, Beltsville, MD, United States.
² Arm & Hammer Animal and Food Production, Waukesha, WI, United States.
³ Department of Pediatrics, University of Maryland School of Medicine, Baltimore, MD, United States.

Abstract

Direct-fed microbials (DFMs) are dietary supplements containing live microorganisms which confer a performance and health benefit to the host, but the mechanisms are unclear. Here, a metabolomics approach was used to identify changes in intestinal metabolite levels in chickens fed an unsupplemented diet or a diet supplemented with B. subtilis strain 1781 or strain 747. Body weight gains of chickens fed the B. subtilis-supplemented diets were increased up to 5.6% in the B. subtilis 1781 group and 7.6% in the B. subtilis 747 group compared with chickens fed the unsupplemented diet. Compared with unsupplemented controls, the levels of 83 metabolites were altered (p < 0.05) (25 increased, 58 decreased) in chickens given the B. subtilis 1781-supplemented diet, while 50 were altered (p < 0.05) (12 increased, 38 decreased) with the B. subtilis 747-supplemented diet. Twenty-two metabolites were altered (p < 0.05) (18 increased, 4 decreased) in the B. subtilis 1781 vs. B. subtilis 747 groups. A random forest analysis of the B. subtilis 1781 vs. control groups gave a predictive accuracy of 87.5%, while that of the B. subtilis 747 vs. control groups was 62.5%. A random forest analysis of the B. subtilis 1781 vs. B. subtilis 747 groups gave a predictive accuracy of 75.0%. Changes in the levels of these intestinal biochemicals provided a distinctive biochemical signature unique to each B. subtilis-supplemented group, and were characterized by alterations in the levels of dipeptides (alanylleucine, glutaminylleucine, phenylalanylalanine, valylglutamine), nucleosides (N1-methyladenosine, N6-methyladenosine, guanine, 2-deoxyguanosine), fatty acids (sebacate, valerylglycine, linoleoylcholine), and carbohydrates (fructose). These results provide the foundation for future studies to identify biochemicals that might be used to improve poultry growth performance in the absence of antibiotic growth promoters.

Keywords: amino acid; fatty acid; gut; metabolomics; nucleoside; probiotics.