Hot air stream reduces cytotoxicity of light-cured calcium hydroxide based cements

Celso-Afonso Klein-Junior; Roberto Zimmer; Diana-Lina-Bronca Borghetti; Fernando-Freitas Portella; Flávia-Carolina Abich; Daniel-Rodrigo Marinowic; Keiichi Hosaka; Eduardo-Galia Reston

doi:10.4317/jced.56590

Hot air stream reduces cytotoxicity of light-cured calcium hydroxide based cements

J Clin Exp Dent. 2020 Mar 1;12(3):e215-e219. doi: 10.4317/jced.56590. eCollection 2020 Mar.

Authors

Celso-Afonso Klein-Junior¹, Roberto Zimmer², Diana-Lina-Bronca Borghetti², Fernando-Freitas Portella³, Flávia-Carolina Abich¹, Daniel-Rodrigo Marinowic⁴, Keiichi Hosaka⁵, Eduardo-Galia Reston²

Affiliations

¹ Department of Operative Dentistry, School of Dentistry, Lutheran University of Brazil (ULBRA), Cachoeira do Sul, Rio Grande do Sul, Brazil.
² Department of Operative Dentistry, School of Dentistry, Lutheran University of Brazil (ULBRA), Canoas, Rio Grande do Sul, Brazil.
³ Institute of Health Sciences, Universidade Feevale, ERS-239 2755, Novo Hamburgo, Rio Grande do Sul, Brazil.
⁴ Neuroscience Department, Brain Institute, Neuroscience Laboratory, Pontifical Catholic University of Rio Grande do Sul, Rio Grande do Sul, Brazil.
⁵ Department of Oral Health Science, School of Medical and Dental Science, Tokyo Medical and Dental University, Tokyo, Japan.

Abstract

Background: The light-cured calcium hydroxide based cements have incomplete polymerization and unconverted monomers can cause pulp cell damage. The aim of this study was to evaluate the influence of a warm and hot air stream on the cytotoxicity of light-cured calcium hydroxide based cements.

Material and methods: The materials Dycal (conventional cement), Biocal, Hidrox-Cal, and Ultra-Blend Plus (light-cured calcium hydroxide cements) were submitted to cytotoxicity analysis after polymerization, without vs. with previous heat treatment with a warm (37°C) and a hot (60°C) air stream. Following polymerization, cements were maintained in culture medium for 24 hours and 7 days, and subjected to the MTT test. Data were analyzed using analysis of variance (ANOVA) followed by post-hoc Student-Newman-Keuls (<0.05).

Results: The results indicated significant differences between the materials according to their composition, i.e., light-cured cements treated with a jet of warm air showed similar cytotoxicity levels to those observed for conventional cement, suggesting that they may be considered alternatives in cases requiring pulp-capping treatment.

Conclusions: Application of a hot air stream reduced cytotoxicity of materials tested. Key words:Dental pulp capping, dental cements, calcium hydroxide, cell survival.