Abstract
We establish a near-infrared two-photon fluorescent probe for the detection of CE2 with high selectivity and sensitivity. This probe exhibits low cytotoxicity and superior tissue penetration ability for evaluating the real-time activity of CE2 in living cells, in cancer tissues, and in a colon carcinoma mice model.
MeSH terms
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Animals
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Antineoplastic Agents / chemistry*
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Antineoplastic Agents / therapeutic use*
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Carboxylesterase / chemistry*
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Carboxylesterase / therapeutic use*
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Carboxylesterase / ultrastructure
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Colonic Neoplasms / diagnostic imaging
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Colonic Neoplasms / drug therapy*
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Female
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Fluorescent Dyes
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Hep G2 Cells
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Humans
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Hydrogen-Ion Concentration
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Magnetic Resonance Spectroscopy
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Mice
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Optical Imaging
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Photons
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Spectroscopy, Near-Infrared
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Xenograft Model Antitumor Assays
Substances
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Antineoplastic Agents
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Fluorescent Dyes
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CES2 protein, human
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Carboxylesterase
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Ces2c protein, mouse