Abstract
The tough challenges for the sensitive and accurate detection of circulating miRNAs (cmiRNAs) in the peripheral blood are low abundance, and high interference from environmental or background factors or other molecules. Here, we developed a double-signal mode based on metal-organic framework coupled cascaded nucleic acid circuits for the accurate and sensitive detection of serum circulating miRNAs.
MeSH terms
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Biosensing Techniques / methods*
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Circulating MicroRNA / blood*
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Circulating MicroRNA / genetics
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DNA / chemistry*
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DNA / genetics
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DNA Probes / chemistry*
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DNA Probes / genetics
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Fluorescent Dyes / chemistry
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Glucose Oxidase / chemistry
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Humans
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Hydrogen Peroxide / chemistry
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Limit of Detection
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Metal-Organic Frameworks / chemistry*
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MicroRNAs / blood
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MicroRNAs / genetics
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Nucleic Acid Hybridization
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Oxidation-Reduction
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Phenazines / chemistry
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Phenylenediamines / chemistry
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Spectrometry, Fluorescence / methods
Substances
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Circulating MicroRNA
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DNA Probes
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Fluorescent Dyes
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MIRN122 microRNA, human
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MIRN21 microRNA, human
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Metal-Organic Frameworks
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MicroRNAs
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Phenazines
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Phenylenediamines
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mirnlet7 microRNA, human
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2,3-diaminophenazine
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1,2-diaminobenzene
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DNA
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Hydrogen Peroxide
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Glucose Oxidase