Neuroblastoma SH-SY5Y cytotoxicity, anti-amyloidogenic activity and cyclooxygenase inhibition of Lasianthus trichophlebus (Rubiaceae)

Mario A Tan; Mark Wilson D Lagamayo; Grecebio Jonathan D Alejandro; Seong Soo A An

doi:10.1007/s13205-020-2145-2

Neuroblastoma SH-SY5Y cytotoxicity, anti-amyloidogenic activity and cyclooxygenase inhibition of Lasianthus trichophlebus (Rubiaceae)

3 Biotech. 2020 Apr;10(4):152. doi: 10.1007/s13205-020-2145-2. Epub 2020 Mar 3.

Authors

Mario A Tan^{1

2

3

4}, Mark Wilson D Lagamayo¹, Grecebio Jonathan D Alejandro^{1

2

3}, Seong Soo A An⁴

Affiliations

¹ 1The Graduate School, University of Santo Tomas, Manila, Philippines.
² 2College of Science, University of Santo Tomas, Manila, Philippines.
³ 3Research Center for the Natural and Applied Sciences, University of Santo Tomas, Manila, Philippines.
⁴ 4Department of BionanoTechnology, Bionano Research Institute, Gachon University, Seongnam, South Korea.

Abstract

The anti-amyloidogenic potential and cyclooxygenase anti-inflammatory activity of Lasianthus trichophlebus extracts were evaluated. The MeOH extract (LTM) and chloroform extract (LTC) exhibited significant cytotoxic inhibition against the neuroblastoma SH-SY5Y cell with an IC₅₀ of 17.52 μg/mL and 12.28 μg/mL, respectively. Thioflavin T assay indicated the LTC extract inhibition (70.56% at 50 μg/mL) to be statistically comparable (p < 0.05) to the positive control. Cyclooxygenase inhibition against COX-1 and COX-2 enzymes gave IC₅₀ values for the LTM extract to be 18.20 and 29.60 µg/mL, respectively; while, the LTC extract showed 4.11 and 2.78 µg/mL, respectively. LC-MS of the LTM extract identified 22 putative compounds, which may prove to be pharmacologically relevant. This study has provided potential insights into the utilization of L. trichophlebus to develop safer plant-based agents for anti-inflammatory or neurodegenerative diseases.

Keywords: Cyclooxygenase; Cytotoxicity; Lasianthus; Neuroblastoma.

Publication types

Case Reports