Elevated conversion of CO2 to versatile formate by a newly discovered formate dehydrogenase from Rhodobacter aestuarii

Abstract

Due to climate change, recent research interests have increased towards CO₂ utilization as a strategy to mitigate the atmospheric CO₂ level. Herein, we aimed to explore formate dehydrogenases (FDHs) from chemoautotroph to discover an efficient and O₂-tolerant biocatalyst for catalyzing the CO₂ reduction to a versatile formate. Through genome-mining and phylogenetic analysis, the FDH from Rhodobacter aestuarii (RaFDH) was newly discovered as a promising O₂-tolernat CO₂ reductase and was successfully expressed in Escherichia coli. In this study, the optimum conditions and turnover rates of RaFDH were examined for CO₂ reduction and formate oxidation. In particular, the RaFDH-driven CO₂ reduction far surpassed the formate oxidation with a turnover rate of 48.3 and 15.6 min^-1, respectively. The outstanding superiority of RaFDH towards CO₂ reduction can be applicable for constructing a feasible electroenzymatic system that produce a versatile formate from CO₂ as a cheap, abundant, and renewable resource.

Keywords: CO(2) reductase; CO(2) reduction; Formate; Rhodobacter aestuarii.