Tumors are characterized by metabolic dysregulation, reprogramming, and the presence of metabolites, which can act both as energy mediators and signaling messengers. Measuring the concentration and composition of metabolites in the tumor microenvironment can help to better understand the tumor pathology and might improve therapeutic treatments. Metabolomics can provide a description of the physiological and pathological status, as well as help to identify biomarkers of the disease. Additionally, mass spectrometry-based tissue imaging techniques can show the spatial distribution of metabolites. In this chapter we present protocols for the extraction and analysis of metabolites and lipids, with emphasis on liquid chromatography-mass spectrometry and mass spectrometry imaging.
Keywords: Lipid; Lipidomics; Metabolite; Metabolomics; Targeted; Tissue imaging; Untargeted.
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