Heavy metal pollution is becoming an increasingly serious problem globally, and cadmium pollution ranks first in the world. Reproduction in insects is affected by cadmium stress in a dose-dependent manner. However, no previous studies have examined the molecular mechanisms underlying the influence of cadmium exposure on insect reproduction. In this study, RNA-Seq was used to investigate changes in ovary gene expression in newly emerged female beet army worms. The beet armyworms were reared under 4 cadmium concentrations: 0 mg/kg (control), low 0.2 mg/kg (L), medium 12.8 mg/kg (M) and high 51.2 mg/kg (H). Compared with the control (CK), a total of 3453 differentially expressed genes (DEGs) were identified in L cadmium stress, including 1791 up-regulated and 1662 down-regulated candidates; in L versus M groups, 982 up-regulated and 658 down-regulated DEGs; and in M versus H groups, 6508 up-regulated and 2000 down-regulated DEGs were identified and the expression patterns of ten genes were verified by q PCR. Many of the identified DEGs were relevant to juvenile hormone and molting hormone biosynthesis, insulin secretion, estrogen signaling, amino acid metabolism and lipid biosynthesis. These data will provide a molecular prospective to understand the ecological risk of heavy metal pollution and are a resource for selecting key genes as targets in gene-editing/silencing technologies for sustainable pest management.
Keywords: Beet armyworm; Cadmium; Heavy metal pollution; Ovary; RNA-Seq; Reproduction.
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