An elegant technology for ultrasensitive impedimetric and voltammetric determination of cholestanol based on a novel molecularly imprinted electrochemical sensor

Ali R Jalalvand; Mohammad Mahdi Zangeneh; Faramarz Jalili; Shokoufeh Soleimani; Jose Manuel Díaz-Cruz

doi:10.1016/j.chemphyslip.2020.104895

An elegant technology for ultrasensitive impedimetric and voltammetric determination of cholestanol based on a novel molecularly imprinted electrochemical sensor

Chem Phys Lipids. 2020 Jul:229:104895. doi: 10.1016/j.chemphyslip.2020.104895. Epub 2020 Mar 9.

Authors

Ali R Jalalvand¹, Mohammad Mahdi Zangeneh², Faramarz Jalili³, Shokoufeh Soleimani³, Jose Manuel Díaz-Cruz⁴

Affiliations

¹ Research Center of Oils and Fats, Research Institute for Health Technology, Kermanshah University of Medical Sciences, Kermanshah, Iran. Electronic address: ali.jalalvand1984@gmail.com.
² Department of Clinical Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran; Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran.
³ Research Center of Oils and Fats, Research Institute for Health Technology, Kermanshah University of Medical Sciences, Kermanshah, Iran.
⁴ Departament d'Enginyeria Química i Química Analítica, Facultat de Química, Universitat de Barcelona, Martí i Franques 1-11, E-8028 Barcelona, Spain.

PMID: 32165169
DOI: 10.1016/j.chemphyslip.2020.104895

Abstract

In this work, a novel molecularly imprinted electrochemical sensor (MIES) has been fabricated based on electropolymerization of a molecularly imprinted polymer (MIP) onto a glassy carbon electrode (GCE) modified with gold-palladium alloy nanoparticles (AuPd NPs)/polydopamine film (PDA)/multiwalled carbon nanotubes-chitosan-ionic liquid (MWCNTs-CS-IL) for voltammetric and impedimetric determination of cholestanol (CHO). Modifications applied to the bare GCE formed an excellent biocompatible composite film which was able to selectively detect CHO molecules. Modifications applied to the bare GCE were characterized by scanning electron microscopy (SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (SEM). Under optimal experimental conditions, the sensor was able to detect CHO in the range of 0.1-60 pM and 1-50 pM by EIS and DPV, respectively. Moreover, the sensor showed high sensitivity, selectivity, repeatability, reproducibility, low interference and good stability towards CHO determination. Our records confirmed that the sensor was successfully able to the analysis real samples for determination of CHO.

Keywords: Cholestanol; Electrochemical sensor; Molecularly imprinted polymer.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biosensing Techniques
Cholestanol / analysis*
Electrochemical Techniques / instrumentation*
Electrochemical Techniques / methods*
Limit of Detection
Molecular Imprinting*

Substances

Cholestanol