Complex study of glyphosate and metabolites influence on enzymatic activity and microorganisms association in soil enriched with Pseudomonas fluorescens and sewage sludge

Piotr Kaczynski; Bozena Lozowicka; Elzbieta Wolejko; Piotr Iwaniuk; Rafal Konecki; Wojciech Dragowski; Jakub Lozowicki; Nurlan Amanbek; Julia Rusilowska; Aleksandra Pietraszko

doi:10.1016/j.jhazmat.2020.122443

Complex study of glyphosate and metabolites influence on enzymatic activity and microorganisms association in soil enriched with Pseudomonas fluorescens and sewage sludge

J Hazard Mater. 2020 Jul 5:393:122443. doi: 10.1016/j.jhazmat.2020.122443. Epub 2020 Mar 2.

Authors

Affiliations

¹ Institute of Plant Protection-National Research Institute, Chelmonskiego 22 Street, 15-195 Bialystok, Poland. Electronic address: p.kaczynski@iorpib.poznan.pl.
² Institute of Plant Protection-National Research Institute, Chelmonskiego 22 Street, 15-195 Bialystok, Poland; Kazakh National Agrarian University, Abai Avenue 8, Almaty, 050010, Kazakhstan.
³ Bialystok University of Technology, Department of Chemistry, Biology and Biotechnology, Wiejska 45A Street, 15-351, Bialystok, Poland. Electronic address: e.wolejko@pb.edu.pl.
⁴ Institute of Plant Protection-National Research Institute, Chelmonskiego 22 Street, 15-195 Bialystok, Poland; University of Bialystok, Department of Microbiology and Biotechnology, Faculty of Biology, Ciolkowskiego 1J Street, 15-245, Bialystok, Poland.
⁵ Institute of Plant Protection-National Research Institute, Chelmonskiego 22 Street, 15-195 Bialystok, Poland.
⁶ Hydratec, Radziwonika 12 Street, 15 -193, Bialystok, Poland.
⁷ Kazakh National Agrarian University, Abai Avenue 8, Almaty, 050010, Kazakhstan.

PMID: 32163800
DOI: 10.1016/j.jhazmat.2020.122443

Abstract

This is the first large scale study of fate of the glyphosate (GLP) and its metabolites, (AMPA, N-acetyl glyphosate, N-acetyl AMPA, sarcosine and glycine) monitored by LC/MS/MS. The laboratory trials of behavior of GLP in two types of agricultural soil were performed. Soil (S), soil enriched with sewage sludge (S + SL), soil with Pseudomonas fluorescens (S + P) and soil enriched with sewage sludge and P. fluorescens (S + SL + P) was treated with Roundup 360 SL under controlled conditions. The presence of metabolites was depended on the soil type and enrichment with sludge or bacteria. The GLP and its soil metabolites caused increase of microorganisms association in comparison to control. We assumed that P. fluorescens and sewage sludge influence on time of GLP dissipation. Moreover, GLP degradation in presence of P. fluorescens and sewage sludge is carried out in different metabolic pathways compared to control (S + GLP). Furthermore, presence of particular GLP metabolites is related to different metabolic pathways and is connected with P. fluorescens and sewage sludge occurrence in soil. Additionally, P. fluorescens and sewage sludge stimulate enzymatic activity of soils.

Keywords: Enzymatic activity; Glyphosate; Metabolites; Pseudomonas fluorescens; Sewage sludge.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acid Phosphatase / chemistry
Alkaline Phosphatase / chemistry
Catalase / chemistry
Glycine / analogs & derivatives*
Glycine / metabolism
Glyphosate
Herbicides / metabolism*
Oxidoreductases / chemistry
Pseudomonas fluorescens / metabolism*
Sewage / microbiology
Soil / chemistry*
Soil Microbiology*
Soil Pollutants / metabolism*

Substances

Herbicides
Sewage
Soil
Soil Pollutants
Oxidoreductases
Catalase
Alkaline Phosphatase
Acid Phosphatase
Glycine