Methods of Generation of Induced Pluripotent Stem Cells and Their Application for the Therapy of Central Nervous System Diseases

E A Cherkashova; G E Leonov; D D Namestnikova; A A Solov'eva; I L Gubskii; T B Bukharova; L V Gubskii; D V Goldstein; K N Yarygin

doi:10.1007/s10517-020-04754-4

Methods of Generation of Induced Pluripotent Stem Cells and Their Application for the Therapy of Central Nervous System Diseases

Bull Exp Biol Med. 2020 Feb;168(4):566-573. doi: 10.1007/s10517-020-04754-4. Epub 2020 Mar 10.

Authors

E A Cherkashova¹, G E Leonov², D D Namestnikova³, A A Solov'eva¹, I L Gubskii¹, T B Bukharova⁴, L V Gubskii^{1

3}, D V Goldstein⁴, K N Yarygin^{5

6}

Affiliations

¹ Federal Center for Cerebrovascular Pathology and Stroke, Ministry of Health of Russian Federation, Moscow, Russia.
² N. P. Bochkov Research Center for Medical Genetics, Moscow, Russia. golerus@gmail.com.
³ N. I. Pirogov Russian National Research Medical University, Ministry of Health of Russian Federation, Moscow, Russia.
⁴ N. P. Bochkov Research Center for Medical Genetics, Moscow, Russia.
⁵ V. N. Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia.
⁶ Russian Medical Academy for Continuous Professional Education, Ministry of Health of the Russian Federation, Moscow, Russia.

PMID: 32157511
DOI: 10.1007/s10517-020-04754-4

Abstract

The use of induced pluripotent stem cells (IPSC) is a promising approach to the therapy of CNS diseases. The undeniable advantage of IPSC technology is the possibility of obtaining practically all types of somatic cells for autologous transplantation bypassing bioethical problems. The review presents integrative and non-integrative methods for obtaining IPSC and the ways of their in vitro and in vivo application for the study and treatment of neurological diseases.

Keywords: cell therapy; induced pluripotent stem cells; ischemic stroke; neural progenitor cells; neurological diseases.

Publication types

Review

MeSH terms

Animals
Cell Differentiation
Genetic Vectors / chemistry
Genetic Vectors / metabolism
Humans
Induced Pluripotent Stem Cells / cytology
Induced Pluripotent Stem Cells / metabolism
Induced Pluripotent Stem Cells / transplantation*
Kruppel-Like Factor 4
Kruppel-Like Transcription Factors / genetics
Kruppel-Like Transcription Factors / metabolism
Lentivirus / genetics
Lentivirus / metabolism
Neural Stem Cells / cytology
Neural Stem Cells / metabolism
Neural Stem Cells / transplantation*
Neurodegenerative Diseases / genetics
Neurodegenerative Diseases / metabolism
Neurodegenerative Diseases / pathology
Neurodegenerative Diseases / therapy*
Octamer Transcription Factor-3 / genetics
Octamer Transcription Factor-3 / metabolism
Precision Medicine / methods*
Proto-Oncogene Proteins c-myc / genetics
Proto-Oncogene Proteins c-myc / metabolism
Retroviridae / genetics
Retroviridae / metabolism
SOXB1 Transcription Factors / genetics
SOXB1 Transcription Factors / metabolism
Stem Cell Transplantation / methods*
Transduction, Genetic / methods

Substances

Kruppel-Like Factor 4
Kruppel-Like Transcription Factors
MYC protein, human
Octamer Transcription Factor-3
POU5F1 protein, human
Proto-Oncogene Proteins c-myc
SOX2 protein, human
SOXB1 Transcription Factors