Abstract
Thrombin, a major protein involved in the clotting cascade by the conversion of inactive fibrinogen to fibrin, plays a crucial role in the development of thrombosis. Antithrombin nanoparticles enable site-specific anticoagulation without increasing bleeding risk. Here we outline the process of making and the characterization of bivalirudin and D-phenylalanyl-L-prolyl-L-arginyl-chloromethyl ketone (PPACK) nanoparticles. Additionally, the characterization of these nanoparticles, including particle size, zeta potential, and quantification of PPACK/bivalirudin loading, is also described.
Keywords:
Anticoagulation; Bivalirudin; PPACK; Perfluorocarbon nanoparticles; Thrombin.
MeSH terms
-
Amino Acid Chloromethyl Ketones / chemical synthesis*
-
Amino Acid Chloromethyl Ketones / chemistry
-
Amino Acid Chloromethyl Ketones / pharmacology
-
Antithrombins / chemical synthesis*
-
Antithrombins / chemistry
-
Antithrombins / pharmacology
-
Fluorocarbons / chemistry*
-
Hirudins / chemical synthesis*
-
Hirudins / chemistry
-
Hirudins / pharmacology
-
Magnetic Iron Oxide Nanoparticles
-
Nanoparticles
-
Particle Size
-
Peptide Fragments / chemical synthesis*
-
Peptide Fragments / chemistry
-
Peptide Fragments / pharmacology
-
Polyhydroxyethyl Methacrylate
-
Recombinant Proteins / chemical synthesis
-
Recombinant Proteins / chemistry
-
Recombinant Proteins / pharmacology
Substances
-
Amino Acid Chloromethyl Ketones
-
Antithrombins
-
Fluorocarbons
-
Hirudins
-
Peptide Fragments
-
Recombinant Proteins
-
Polyhydroxyethyl Methacrylate
-
phenylalanyl-prolyl-arginine-chloromethyl ketone
-
bivalirudin