Treatment of Intervertebral Disc Degeneration in Wistar Rats with Mesenchymal Stem Cells

A P Lykov; N A Bondarenko; O V Poveshchenko; I I Kim; M A Surovtseva; J B Sadykova; P A Semin; E L Zavjalov; A L Krivoshapkin; V I Konenkov

doi:10.1007/s10517-020-04756-2

Treatment of Intervertebral Disc Degeneration in Wistar Rats with Mesenchymal Stem Cells

Bull Exp Biol Med. 2020 Feb;168(4):578-582. doi: 10.1007/s10517-020-04756-2. Epub 2020 Mar 9.

Authors

Affiliations

¹ Research Institute of Clinical and Experimental Lymphology, Affiliated Branch of Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia. aplykov2@mail.ru.
² Research Institute of Clinical and Experimental Lymphology, Affiliated Branch of Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.

PMID: 32152846
DOI: 10.1007/s10517-020-04756-2

Abstract

We studied the effect of erythropoietin on functional properties of mesenchymal stem cells under conditions of oxidative stress and their therapeutic potential in the treatment of intervertebral disc degeneration in Wistar rats. It was shown that erythropoietin stimulates proliferation under conditions of oxidative stress. Injection of bone marrow mesenchymal stem cells into the damaged intervertebral disc was followed by an increase in the height of the intervertebral disc and activation of repair processes in the nucleus pulposus. The combination of mesenchymal stem cells with erythropoietin provides the best effect of cell therapy in case of intervertebral disc damage.

Keywords: erythropoietin; intervertebral disc puncture; mesenchymal stem cells; oxidative stress.

MeSH terms

Animals
Bone Marrow Cells / cytology
Bone Marrow Cells / drug effects*
Bone Marrow Cells / metabolism
Cell Proliferation / drug effects
Disease Models, Animal
Erythropoietin / pharmacology*
Femur / cytology
Humans
Hydrogen Peroxide / antagonists & inhibitors
Hydrogen Peroxide / pharmacology
Injections, Intralesional
Intervertebral Disc / drug effects*
Intervertebral Disc / injuries
Intervertebral Disc / metabolism
Intervertebral Disc Degeneration / metabolism
Intervertebral Disc Degeneration / pathology
Intervertebral Disc Degeneration / therapy*
Male
Mesenchymal Stem Cell Transplantation*
Mesenchymal Stem Cells / cytology
Mesenchymal Stem Cells / drug effects*
Mesenchymal Stem Cells / metabolism
Oxidative Stress
Rats
Rats, Wistar

Substances

Erythropoietin
Hydrogen Peroxide