Integrated analysis of the transcriptome and metabolome of purple and green leaves of Tetrastigma hemsleyanum reveals gene expression patterns involved in anthocyanin biosynthesis

PLoS One. 2020 Mar 9;15(3):e0230154. doi: 10.1371/journal.pone.0230154. eCollection 2020.

Abstract

To gain better insight into the regulatory networks of anthocyanin biosynthesis, an integrated analysis of the metabolome and transcriptome in purple and green leaves of Tetrastigma hemsleyanum was conducted. Transcript and metabolite profiles were archived by RNA-sequencing data analysis and LC-ESI-MS/MS, respectively. There were 209 metabolites and 4211 transcripts that were differentially expressed between purple and green leaves. Correlation tests of anthocyanin contents and transcriptional changes showed 141 significant correlations (Pearson correlation coefficient >0.8) between 16 compounds and 14 transcripts involved in the anthocyanin biosynthesis pathway. Some novel genes and metabolites were discovered as potential candidate targets for the improvement of anthocyanin content and superior cultivars.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anthocyanins / genetics
  • Anthocyanins / metabolism*
  • Color
  • Computational Biology
  • Gene Expression Profiling
  • Gene Expression Regulation, Plant
  • Genes, Plant
  • Metabolome / genetics*
  • Plant Leaves / metabolism*
  • Plant Proteins / genetics
  • Tandem Mass Spectrometry
  • Transcriptome / genetics*
  • Vitaceae / chemistry
  • Vitaceae / genetics*
  • Vitaceae / metabolism

Substances

  • Anthocyanins
  • Plant Proteins

Grants and funding

This work was sponsored by grants Active Design Project of Hangzhou Agricultural and Social Development Scientific Research (No. 20190101A05), and Science and Technology Innovation and Demonstration extension Fund of Hangzhou Academy of Agricultural Sciences (No. 2019HNCT-31).