Multiple PCR (Multiplex polymerase chain reaction, MPCR) is a technology to simultaneously amplify multiple targets through a single reaction and to detect the amplification products by reliable detection means so as to realize the diagnosis of multiple targets. MPCR has been well studied for its high efficiency, high throughput and low cost. At present, MPCR has been widely used in scientific research, disease diagnosis and other fields. Here, we summarize the development and application of MPCR from amplification and detection, and discuss the advantages and existing problems of MPCR. We propose that separate the reaction mixture into droplets or combined MPCR with the capillary convective PCR is expect to further improve the amplification efficiency of the surface of the solid phase carrier, so as to provide reference for the development of multiple PCR with high amplification efficiency, good consistency, good stability and multiplex detection.
多重PCR (Multiplex polymerase chain reaction，MPCR) 是指通过一次PCR 反应同时对多个靶标进行扩增，结合一定的检测手段对扩增产物进行检测从而实现对多个靶标进行诊断的技术。MPCR 具有高效率、高通量、低成本的特性而被深入研究。目前MPCR 技术已被广泛应用于科学研究和疾病诊断等领域，文中从MPCR 扩增与检测两方面概述了MPCR 技术发展及其应用，讨论了MPCR 技术的优点及存在的问题，并且提出将反应体系分隔成小液滴或结合管式对流PCR (Capillary convective PCR，CCPCR) 技术的方式有望提高固相载体表面的扩增效率，从而开发出扩增效率高、一致性好、体系稳定、检测重数高的多重PCR 技术。.
Keywords: Luminex liquid chip; melting curve; membrane chromatography; microfluidic; multiple PCR.