Automated Versus Manual Band Counts for the Diagnosis of Invasive Bacterial Infections in Infants Who Are Febrile

Sriram Ramgopal; Paria M Wilson

doi:10.1016/j.jpeds.2020.01.065

Automated Versus Manual Band Counts for the Diagnosis of Invasive Bacterial Infections in Infants Who Are Febrile

J Pediatr. 2020 Jun:221:246-250.e3. doi: 10.1016/j.jpeds.2020.01.065. Epub 2020 Mar 4.

Authors

Sriram Ramgopal¹, Paria M Wilson²

Affiliations

¹ Division of Emergency Medicine, Department of Pediatrics, Ann and Robert H. Lurie Children's Hospital of Chicago, Feinberg School of Medicine, Chicago, IL. Electronic address: sramgopal@luriechildrens.org.
² Department of Pediatrics, University of Cincinnati, College of Medicine, Cincinnati, OH; Division of Emergency Medicine, Department of Pediatrics, Cincinnati Children's Hospital Medical Center, Cincinnati, OH.

PMID: 32145966
DOI: 10.1016/j.jpeds.2020.01.065

Abstract

We conducted a secondary analysis of a prospective study of infants ≤60 days of age who were febrile to assess the diagnostic accuracy of automated vs manual immature neutrophils for invasive bacterial infections. Although manual counts were superior compared with automated counts, bands had suboptimal accuracy overall and had significant variability in test characteristics based on methodology.

Keywords: IBI; febrile infant; granulocytes; immature neutrophils; invasive bacterial infection; sepsis.

Publication types

Comparative Study
Multicenter Study
Observational Study

MeSH terms

Autoanalysis
Bacterial Infections / diagnosis*
Blood Cell Count / methods*
Datasets as Topic
Emergency Service, Hospital
Female
Fever / microbiology
Humans
Infant
Infant, Newborn
Leukocyte Count*
Male
Neutrophils / cytology*
Predictive Value of Tests
Prospective Studies
Sampling Studies
Sensitivity and Specificity