Detection of melon necrotic spot virus by one-step reverse transcription loop-mediated isothermal amplification assay

PLoS One. 2020 Mar 5;15(3):e0230023. doi: 10.1371/journal.pone.0230023. eCollection 2020.

Abstract

Melon necrotic spot virus (MNSV) can cause significant economic losses due to decreased quality in cucurbit crops. The current study is the first to use reverse transcription loop-mediated isothermal amplification (RT-LAMP) for detection of MNSV. A set of four LAMP primers was designed based on the coat protein gene sequence of MNSV, and a RT-LAMP reaction was successfully performed for 1 h at 62°C. The results of RT-LAMP showed high specificity for MNSV and no cross-reaction with other viruses. Compared to traditional reverse transcription-PCR (RT-PCR), the RT-LAMP assay was 103-fold more sensitive in detecting MNSV. Due to its sensitivity, speed and visual assessment, RT-LAMP is appropriate for detecting MNSV in the laboratory.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Nucleic Acid Amplification Techniques*
  • Reverse Transcription*
  • Time Factors
  • Tombusviridae / genetics*
  • Tombusviridae / isolation & purification*

Supplementary concepts

  • Melon necrotic spot virus

Grants and funding

This research has been supported by the following grants: Program for Applied Agricultural Technology Innovation of Shandong (No. SD2019ZZ004), Shandong Provincial Natural Science Foundation, China (No. ZR2017CM058), the Science and Technology Development Planning Program of Weifang (No. 2017GX076), and the Scientific Research Project of Facility Horticulture Laboratory of Universities in Shandong (No. 2018YY002).