Effects of Gamma-Radiation on DNA Damage in Onion (Allium cepa L.) Seedlings

A Ya Bolsunovsky; D V Dementyev; T S Frolova; E A Trofimova; E M Iniatkina; S A Vasilyev; O I Sinitsyna

doi:10.1134/S1607672919060024

Effects of Gamma-Radiation on DNA Damage in Onion (Allium cepa L.) Seedlings

Dokl Biochem Biophys. 2019 Nov;489(1):362-366. doi: 10.1134/S1607672919060024. Epub 2020 Mar 4.

Authors

A Ya Bolsunovsky¹, D V Dementyev², T S Frolova^{3

4}, E A Trofimova², E M Iniatkina², S A Vasilyev⁵, O I Sinitsyna^{3

4}

Affiliations

¹ Institute of Biophysics, Krasnoyarsk Science Center, Siberian Branch, Russian Academy of Sciences, 660036, Krasnoyarsk, Russia. radecol@ibp.ru.
² Institute of Biophysics, Krasnoyarsk Science Center, Siberian Branch, Russian Academy of Sciences, 660036, Krasnoyarsk, Russia.
³ Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, 630090, Novosibirsk, Russia.
⁴ Novosibirsk State University, 630090, Novosibirsk, Russia.
⁵ Research Institute of Medical Genetics, Tomsk National Research Medical Center, Russian Academy of Sciences, 634058, Tomsk, Russia.

PMID: 32130600
DOI: 10.1134/S1607672919060024

Abstract

The effect of γ-radiation on the level of nuclear DNA damage in onion seedlings (Allium-test) was studied using the comet assay. DNA breaks were first found in cells of onion seedlings exposed to low-dose radiation (≤ 0.1 Gy). Dose dependence of DNA damage parameters showed nonlinear behavior: a linear section in the low-dose region (below 0.1 Gy) and a dose-independent plateau in the dose range between 1 and 5 Gy. Thus, the comet assay can be used to estimate the biological effects of low-dose γ-radiation on Allium cepa seedlings.

MeSH terms

DNA Damage*
Dose-Response Relationship, Radiation
Gamma Rays / adverse effects*
Onions / genetics*
Onions / radiation effects*
Seedlings / genetics*
Seedlings / radiation effects*