Cytoprotective Activity of Ethylacetate Fraction of Picria fel-terrae Lour. Herbs

Denny Satria; Jansen Silalahi; Ginda Haro; Syafruddin Ilyas; Poppy Anjelisa Zaitun Hasibuan

doi:10.3889/oamjms.2019.521

Cytoprotective Activity of Ethylacetate Fraction of Picria fel-terrae Lour. Herbs

Open Access Maced J Med Sci. 2019 Nov 14;7(22):3865-3867. doi: 10.3889/oamjms.2019.521. eCollection 2019 Nov 30.

Authors

Denny Satria¹, Jansen Silalahi², Ginda Haro², Syafruddin Ilyas³, Poppy Anjelisa Zaitun Hasibuan⁴

Affiliations

¹ Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia.
² Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia.
³ Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan, Indonesia.
⁴ Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia.

Abstract

Aim: Free radicals produce from metabolism or enviromental which interact continously with biological system. Picria fel-terrae Lour. herbs have been used as antioxidant and treat various diseases. The aim of this study was to evaluate cytoprotective activity of ethylacetate fraction (EAF) of Picria fel-terrae Lour. herbs.

Methods: Cytoprotective activity were determined by MTT asay and flow cytometry assay on Vero cells which induced with H₂O₂ 0.8 mM.

Results: EAF at 100 µg/mL were showed highest viability (88.83 ± 2.90%) and ROS expression (66.75%) on Vero cells.

Conclusion: EAF of Picria fel-terrae Lour. herbs have cytoprotective activity.

Keywords: Cytoprotective; Ethylacetate; Herbs; Picria fel-terrae Lour.