Comparison of Commensal and Clinical Isolates for Diversity of Plasmids in Escherichia coli and Klebsiella pneumoniae

Antimicrob Agents Chemother. 2020 Apr 21;64(5):e02064-19. doi: 10.1128/AAC.02064-19. Print 2020 Apr 21.

Abstract

In this study, the plasmid content of clinical and commensal strains was analyzed and compared. The replicon profile was similar in both populations, except for L, M, A/C, and N (detected only in clinical strains) and HI1 (only in commensal strains). Although I1 and F were the most frequent replicons, only IncI1, sequence type 12 (ST12) was associated with blaCMY-2 in both populations. In contrast, the widespread resistant IncF plasmids were not linked to a single epidemic plasmid.

Keywords: Enterobacteriaceae; antimicrobial resistance; pMLST; plasmid epidemiology; replicon.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics*
  • Drug Resistance, Bacterial / genetics*
  • Escherichia coli / genetics*
  • Escherichia coli / isolation & purification
  • Feces / microbiology
  • Humans
  • Klebsiella pneumoniae / genetics*
  • Klebsiella pneumoniae / isolation & purification
  • Multilocus Sequence Typing
  • Plasmids / genetics*
  • beta-Lactamases / genetics*

Substances

  • Bacterial Proteins
  • beta-lactamase CMY-2
  • beta-Lactamases
  • carbapenemase