Epidemiology and control of emerging criniviruses in bean

Abstract

During the last two decades, new criniviruses emerged in green bean crops in the south-east of Spain. Bean yellow disorder virus (BnYDV) was first detected in 2003 and caused major economic damage in crops grown in greenhouses. It was characterized as the first crinivirus to infect a member species of the Leguminosae family. Symptoms induced during BnYDV infection include interveinal chlorosis and yellowing on leaves, and reduced fruit yield and quality. Similar symptoms, although more severe, were observed in bean crops in the same region during the fall of 2011. From that moment on, BnYDV was not detected anymore in diseased plants, but instead lettuce chlorosis virus (LCV) was associated with the diseased plants. Previously, LCV was detected only in California, USA, infecting lettuce and sugarbeets. The host range and partial genomic sequences lead to the description of the new strain, LCV-SP. The complete sequence of its genome revealed the virus as a recombinant of BnYDV and LCV, in which the latter had lost two ORFs in the RNA1 of the bipartite genome and had acquired two homologue ORFs from BnYDV. Both viruses are transmitted by the whitefly Bemisia tabaci. When compared with other crinivirus pathosystems, the transmission efficiency of BnYDV to its primary host bean, is among the highest, and its persistence in the vector among the longest, up to 9 days. The host range of BnYDV s restricted to several crop species of the Leguminosae: common bean (Phaseolus vulgaris), pea (Pisum sativum), tirabeque (P. sativum subsp. sativum var. macrocarpon), lentil (Lens culinaris) and faba bean (Vicia faba). LCV-SP is also able to infect green bean plants but not lettuce, its original host, probably following its recombinant nature. Symptoms and epidemiology of the bean criniviruses are compared with similar pathosystems that occur in the same region and that involve cucurbit yellow stunting disorder virus and tomato chlorosis virus, infecting cucurbitaceous and solanaceous crops, respectively. Control of the criniviruses in bean crops will depend on efficient control of the vector. Physical control with greenhouses that prevent viruliferous whiteflies from gaining access to crops reduces BnYDV infection in plants and loss of production. Integrated pest management in beans would be preferred and the use of natural enemies to reduce secondary spread within greenhouses must be investigated.

Keywords: Genomic recombination; Host range; Symptoms; Virus emergence; Whitefly.