Early changes in the urine proteome in a rat liver tumour model

Yameng Zhang; Yufei Gao; Youhe Gao

doi:10.7717/peerj.8462

Early changes in the urine proteome in a rat liver tumour model

PeerJ. 2020 Feb 10:8:e8462. doi: 10.7717/peerj.8462. eCollection 2020.

Authors

Yameng Zhang¹, Yufei Gao², Youhe Gao¹

Affiliations

¹ Department of Biochemistry and Molecular Biology, Beijing Normal University, Gene Engineering Drug and Biotechnology Beijing Key Laboratory, Beijing, China.
² College of Information Science and Technology, Beijing Normal University, Beijing, China.

Abstract

Background: Urine, as a potential biomarker source among body fluids, can accumulate many early changes in the body due to the lack of mechanisms to maintain a homeostatic state. This study aims to detect early changes in the urinary proteome in a rat liver tumour model.

Methods: The tumour model was established with the Walker-256 carcinosarcoma cell line (W256). Urinary proteins at days 3, 5, 7 and 11 were profiled by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). Compared with controls, differential proteins were selected. Associations of differential proteins with cancer were retrieved.

Results: At days 3, 5, 7 and 11, five, fifteen, eleven and twelve differential proteins were identified, respectively. Some of the differential proteins were reported to be associated with liver cancer. This differential urinary protein pattern was different from the patterns in W256 subcutaneous, lung metastasis and intracerebral tumour models.

Conclusions: This study demonstrates that (1) early changes in urinary proteins can be found in the rat liver tumour model; (2) urinary proteins can be used to differentiate the same tumour cells grown in different organs.

Keywords: Biomarker; Liver tumour; Proteome; Urine.

Associated data

figshare/10.6084/m9.figshare.8293220.v1

Grants and funding

This work was supported by the National Key Research and Development Program of China (2018YFC0910202 and 2016 YFC 1306300), Beijing Natural Science Foundation (7172076), Beijing cooperative construction project (110651103), Beijing Normal University (11100704), and Peking Union Medical College Hospital (2016-2.27). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.