Tea Polyphenols Inhibit Rat Osteoclast Formation and Differentiation

Yoshiomi Oka; Shinichi Iwai; Hitoshi Amano; Yuko Irie; Kentaro Yatomi; Kakei Ryu; Shoji Yamada; Katsunori Inagaki; Katsuji Oguchi

doi:10.1254/jphs.11082FP

Tea Polyphenols Inhibit Rat Osteoclast Formation and Differentiation

J Pharmacol Sci. 2012;118(1):55-64. doi: 10.1254/jphs.11082FP. Epub 2019 Jun 11.

Authors

Yoshiomi Oka¹, Shinichi Iwai², Hitoshi Amano³, Yuko Irie⁴, Kentaro Yatomi⁴, Kakei Ryu⁴, Shoji Yamada³, Katsunori Inagaki⁵, Katsuji Oguchi⁴

Affiliations

¹ Department of Pharmacology, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan; Department of Orthopaedic Surgery, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
² Department of Pharmacology, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan. Electronic address: shinichi@med.showa-u.ac.jp.
³ Department of Pharmacology, Showa University School of Dentistry, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
⁴ Department of Pharmacology, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
⁵ Department of Orthopaedic Surgery, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.

PMID: 32092838
DOI: 10.1254/jphs.11082FP

Abstract

Matrix metalloproteinases (MMPs) play an important role in degeneration of the matrix associated with bone and cartilage. Regulation of osteoclast activity is essential in the treatment of bone disease, including osteoporosis and rheumatoid arthritis. Polyphenols in green tea, particularly epigallocatechin-3-gallate (EGCG), inhibit MMPs expression and activity. However, the effects of the black tea polyphenol, theaflavin-3,3'-digallate (TFDG), on osteoclast and MMP activity are unknown. Therefore, we examined whether TFDG and EGCG affect MMP activity and osteoclast formation and differentiation in vitro. TFDG or EGCG (10 and 100 μM) was added to cultures of rat osteoclast precursors cells and mature osteoclasts. Numbers of multinucleated osteoclasts and actin rings decreased in polyphenol-treated cultures relative to control cultures. MMP-2 and MMP-9 activities were lower in TFDG- and EGCG-treated rat osteoclast precursor cells than in control cultures. MMP-9 mRNA levels declined significantly in TFDG-treated osteoclasts in comparison to control osteoclasts. TFDG and EGCG inhibited the formation and differentiation of osteoclasts via inhibition of MMPs. TFDG may suppress actin ring formation more effectively than EGCG. Thus, TFDG and EGCG may be suitable agents or lead compounds for the treatment of bone resorption diseases.

Keywords: epigallocatechin-3-gallate (EGCG); matrix metalloproteinase (MMP)-9; rat osteoclast; rat osteoclast precursor cell; theaflavin-3,3′-digallate (TFDG).