Agrobacterium-mediated transformation of arthroconidia obtained from the edible mushroom Hypsizygus marmoreus

Dapeng Bao; Zhiheng Huang; Yan Li; Chenli Zhou; Yan Li; Jianing Wan; Lihua Tang; Wenjun Mao; Ying Wang; Ming Gong; Gen Zou; Yoichi Honda; Ruiheng Yang; Junjun Shang

doi:10.1016/j.mimet.2020.105878

Agrobacterium-mediated transformation of arthroconidia obtained from the edible mushroom Hypsizygus marmoreus

J Microbiol Methods. 2020 Apr:171:105878. doi: 10.1016/j.mimet.2020.105878. Epub 2020 Feb 21.

Authors

Dapeng Bao¹, Zhiheng Huang², Yan Li³, Chenli Zhou³, Yan Li³, Jianing Wan³, Lihua Tang³, Wenjun Mao³, Ying Wang³, Ming Gong³, Gen Zou³, Yoichi Honda⁴, Ruiheng Yang⁵, Junjun Shang⁶

Affiliations

¹ College of Food Science, Shanghai Ocean University, 999 Huchenghuan Road, Pudong District, Shanghai 201306, China; Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, 1000 Jingqi road, Fengxian District, Shanghai 201403, China.
² College of Food Science, Shanghai Ocean University, 999 Huchenghuan Road, Pudong District, Shanghai 201306, China.
³ Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, 1000 Jingqi road, Fengxian District, Shanghai 201403, China.
⁴ Laboratory of Forest Biochemistry, Division of Environmental Science and Technology, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwake-cho, Sakyoku-ku, Kyoto 606-8502, Japan.
⁵ Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, 1000 Jingqi road, Fengxian District, Shanghai 201403, China. Electronic address: yangruiheng@126.com.
⁶ College of Food Science, Shanghai Ocean University, 999 Huchenghuan Road, Pudong District, Shanghai 201306, China; Shanghai Key Laboratory of Agricultural Genetics and Breeding, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, 1000 Jingqi road, Fengxian District, Shanghai 201403, China. Electronic address: shangjunjun@saas.sh.cn.

PMID: 32092329
DOI: 10.1016/j.mimet.2020.105878

Abstract

Using the carboxin resistance gene from Pleurotus eryngii as a selective marker, we introduced foreign DNA into the arthroconidia of Hypsizygus marmoreus through Agrobacterium-mediated transformation. The function of the exogenous GUS (β-glucuronidase) gene driven by the CaMV35S promoter was detected in the transformants.

Keywords: Arthroconidia; Hypsizygus marmoreus; Transformation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Agaricales / genetics*
Agaricales / metabolism
Agrobacterium / genetics*
Carboxin / pharmacology
Drug Resistance, Fungal / genetics
Fungicides, Industrial / pharmacology
Glucuronidase / genetics*
Plasmids / genetics
Pleurotus / genetics
Promoter Regions, Genetic / genetics
Spores, Fungal / genetics*
Transformation, Genetic / genetics*

Substances

Fungicides, Industrial
Carboxin
Glucuronidase

Supplementary concepts

Hypsizygus marmoreus
Pleurotus eryngii