MCSeEd (Methylation Context Sensitive Enzyme ddRAD): A New Method to Analyze DNA Methylation

Marco Di Marsico; Elisa Cerruti; Cinzia Comino; Andrea Porceddu; Alberto Acquadro; Stefano Capomaccio; Gianpiero Marconi; Emidio Albertini

doi:10.1007/978-1-0716-0179-2_4

MCSeEd (Methylation Context Sensitive Enzyme ddRAD): A New Method to Analyze DNA Methylation

Methods Mol Biol. 2020:2093:47-64. doi: 10.1007/978-1-0716-0179-2_4.

Authors

Marco Di Marsico¹, Elisa Cerruti², Cinzia Comino², Andrea Porceddu³, Alberto Acquadro², Stefano Capomaccio⁴, Gianpiero Marconi⁵, Emidio Albertini¹

Affiliations

¹ Department of Agricultural, Food and Environmental Sciences, University of Perugia, Perugia, Italy.
² Department of Agricultural, Forest and Food Sciences, Plant Genetics and Breeding, University of Torino, Grugliasco, Italy.
³ Department of Agriculture, University of Sassari, Sassari, Italy.
⁴ Department of Veterinary Medicine, University of Perugia, Perugia, Italy.
⁵ Department of Agricultural, Food and Environmental Sciences, University of Perugia, Perugia, Italy. gianpiero.marconi@unipg.it.

PMID: 32088888
DOI: 10.1007/978-1-0716-0179-2_4

Abstract

Methylation context sensitive enzyme ddRAD (MCSeEd) is a NGS-based method for genome-wide investigations of DNA methylation at different contexts requiring only low to moderate sequencing depth. It is particularly useful for identifying methylation changes in experimental systems challenged by biotic or abiotic stresses or at different developmental stages.

Keywords: DNA methylation; Development; MCSEeD; Methylation context sensitive enzyme ddRAD; Regulation gene expression; Sequencing; Zea mays.

MeSH terms

DNA Methylation / genetics*
DNA, Plant / genetics
Epigenesis, Genetic / genetics
Genome, Plant / genetics
Stress, Physiological / genetics
Zea mays / genetics*

Substances

DNA, Plant