A metabolite-specific 3D stack-of-spiral bSSFP sequence for improved lactate imaging in hyperpolarized [1-13 C]pyruvate studies on a 3T clinical scanner

Abstract

Purpose: The balanced steady-state free precession sequence has been previously explored to improve the efficient use of nonrecoverable hyperpolarized ¹³C magnetization, but suffers from poor spectral selectivity and long acquisition time. The purpose of this study was to develop a novel metabolite-specific 3D bSSFP ("MS-3DSSFP") sequence with stack-of-spiral readouts for improved lactate imaging in hyperpolarized [1-¹³ C]pyruvate studies on a clinical 3T scanner.

Methods: Simulations were performed to evaluate the spectral response of the MS-3DSSFP sequence. Thermal ¹³C phantom experiments were performed to validate the MS-3DSSFP sequence. In vivo hyperpolarized [1-¹³ C], pyruvate studies were performed to compare the MS-3DSSFP sequence with metabolite-specific gradient echo ("MS-GRE") sequences for lactate imaging.

Results: Simulations, phantom, and in vivo studies demonstrate that the MS-3DSSFP sequence achieved spectrally selective excitation on lactate while minimally perturbing other metabolites. Compared with MS-GRE sequences, the MS-3DSSFP sequence showed approximately a 2.5-fold SNR improvement for lactate imaging in rat kidneys, prostate tumors in a mouse model, and human kidneys.

Conclusions: Improved lactate imaging using the MS-3DSSFP sequence in hyperpolarized [1-¹³ C]pyruvate studies was demonstrated in animals and humans. The MS-3DSSFP sequence could be applied for other clinical applications such as in the brain or adapted for imaging other metabolites such as pyruvate and bicarbonate.

Keywords: MRI; bSSFP; hyperpolarized; lactate; metabolic imaging; pyruvate; ¹³C; 13C.