Real-time effects of PEEP and tidal volume on regional ventilation and perfusion in experimental lung injury

João Batista Borges; John N Cronin; Douglas C Crockett; Göran Hedenstierna; Anders Larsson; Federico Formenti

doi:10.1186/s40635-020-0298-2

Real-time effects of PEEP and tidal volume on regional ventilation and perfusion in experimental lung injury

Intensive Care Med Exp. 2020 Feb 21;8(1):10. doi: 10.1186/s40635-020-0298-2.

Authors

João Batista Borges¹, John N Cronin², Douglas C Crockett³, Göran Hedenstierna⁴, Anders Larsson⁵, Federico Formenti^{6

7}

Affiliations

¹ Centre for Human and Applied Physiological Sciences, King's College London, London, UK. joaobatistaborges8@gmail.com.
² Centre for Human and Applied Physiological Sciences, King's College London, London, UK.
³ Nuffield Division of Anaesthetics, University of Oxford, Oxford, UK.
⁴ Hedenstierna Laboratory, Department of Medical Sciences, Uppsala University, Uppsala, Sweden.
⁵ Hedenstierna Laboratory, Department of Surgical Sciences, Uppsala University, Uppsala, Sweden.
⁶ Centre for Human and Applied Physiological Sciences, King's College London, London, UK. federico.formenti@kcl.ac.uk.
⁷ Nuffield Division of Anaesthetics, University of Oxford, Oxford, UK. federico.formenti@kcl.ac.uk.

Abstract

Background: Real-time bedside information on regional ventilation and perfusion during mechanical ventilation (MV) may help to elucidate the physiological and pathophysiological effects of MV settings in healthy and injured lungs. We aimed to study the effects of positive end-expiratory pressure (PEEP) and tidal volume (V_T) on the distributions of regional ventilation and perfusion by electrical impedance tomography (EIT) in healthy and injured lungs.

Methods: One-hit acute lung injury model was established in 6 piglets by repeated lung lavages (injured group). Four ventilated piglets served as the control group. A randomized sequence of any possible combination of three V_T (7, 10, and 15 ml/kg) and four levels of PEEP (5, 8, 10, and 12 cmH₂O) was performed in all animals. Ventilation and perfusion distributions were computed by EIT within three regions-of-interest (ROIs): nondependent, middle, dependent. A mixed design with one between-subjects factor (group: intervention or control), and two within-subjects factors (PEEP and V_T) was used, with a three-way mixed analysis of variance (ANOVA).

Results: Two-way interactions between PEEP and group, and V_T and group, were observed for the dependent ROI (p = 0.035 and 0.012, respectively), indicating that the increase in the dependent ROI ventilation was greater at higher PEEP and V_T in the injured group than in the control group. A two-way interaction between PEEP and V_T was observed for perfusion distribution in each ROI: nondependent (p = 0.030), middle (p = 0.006), and dependent (p = 0.001); no interaction was observed between injured and control groups.

Conclusions: Large PEEP and V_T levels were associated with greater pulmonary ventilation of the dependent lung region in experimental lung injury, whereas they affected pulmonary perfusion of all lung regions both in the control and in the experimental lung injury groups.

Keywords: Electrical impedance tomography; Mechanical ventilation; Pulmonary circulation; Respiratory distress syndrome, Adult; Ventilation-perfusion ratio.

Abstract

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