Arabinoxylan-co-AA/HAp/TiO2 nanocomposite scaffold a potential material for bone tissue engineering: An in vitro study

Muhammad Umar Aslam Khan; Sajjad Haider; Saqlain A Shah; Saiful Izwan Abd Razak; Shukur Abu Hassan; Mohammed Rafiq Abdul Kadir; Adnan Haider

doi:10.1016/j.ijbiomac.2020.02.142

Arabinoxylan-co-AA/HAp/TiO₂ nanocomposite scaffold a potential material for bone tissue engineering: An in vitro study

Int J Biol Macromol. 2020 May 15:151:584-594. doi: 10.1016/j.ijbiomac.2020.02.142. Epub 2020 Feb 17.

Authors

Muhammad Umar Aslam Khan¹, Sajjad Haider², Saqlain A Shah³, Saiful Izwan Abd Razak⁴, Shukur Abu Hassan⁵, Mohammed Rafiq Abdul Kadir⁶, Adnan Haider⁷

Affiliations

¹ Department of Polymer Engineering and Technology, University of the Punjab, Lahore 54590, Pakistan; School of Biomedical Engineering and Health Sciences, Faculty of Engineering, University Teknologi Malaysia, 81300, Johor, Malaysia; School of Biomedical and Engineering, Shanghai Jiaotong University, Dongchuan Rd 800, 200240 Shanghai, China. Electronic address: umar007khan@gmail.com.
² Department of Chemical Engineering, College of Engineering, King Saud University, P.O. BOX 800, Riyadh 11421, KSA, Saudi Arabia.
³ Nanotechnology & Biomaterials Lab, Physics Department, Forman Christian College University, Lahore 54000, Pakistan.
⁴ School of Biomedical Engineering and Health Sciences, Faculty of Engineering, University Teknologi Malaysia, 81300, Johor, Malaysia; Center for Advanced Composite Materials, University Teknologi Malaysia, Sakudai, Johor, Malaysia.
⁵ Center for Advanced Composite Materials, University Teknologi Malaysia, Sakudai, Johor, Malaysia.
⁶ School of Biomedical Engineering and Health Sciences, Faculty of Engineering, University Teknologi Malaysia, 81300, Johor, Malaysia.
⁷ Department of Biological Sciences, National University of Medical Sciences, Rawalpindi, Punjab, Pakistan. Electronic address: adnan_phd@outlook.com.

PMID: 32081758
DOI: 10.1016/j.ijbiomac.2020.02.142

Abstract

Arabinoxylan (AX) is a natural biological macromolecule with several potential biomedical applications. In this research, AX, nano-hydroxyapatite (n-HAp) and titanium dioxide (TiO₂) based polymeric nanocomposite scaffolds were fabricated by the freeze-drying method. The physicochemical characterizations of these polymeric nanocomposite scaffolds were performed for surface morphology, porosity, swelling, biodegradability, mechanical, and biological properties. The scaffolds exhibited good porosity and rough surface morphology, which were efficiently controlled by TiO₂ concentrations. MC3T3-E1 cells were employed to conduct the biocompatibility of these scaffolds. Scaffolds showed unique biocompatibility in vitro and was favorable for cell attachment and growth. PNS3 proved more biocompatible, showed interconnected porosity and substantial mechanical strength compared to PNS1, PNS2 and PNS4. Furthermore, it has also showed more affinity to cells and cell growth. The results illustrated that the bioactive nanocomposite scaffold has the potential to find applications in the tissue engineering field.

Keywords: Arabinoxylan; Biocompatible; Biopolymer; Nanoparticles; Porous materials.

MeSH terms

Animals
Biocompatible Materials / chemistry*
Biopolymers / chemistry
Bone and Bones*
Cell Line
Cell Survival
Durapatite / chemistry*
Mice
Molecular Structure
Nanocomposites / chemistry*
Nanocomposites / ultrastructure
Porosity
Spectrum Analysis
Stress, Mechanical
Tissue Engineering*
Tissue Scaffolds / chemistry*
Titanium / chemistry*
Xylans / chemistry*

Substances

Biocompatible Materials
Biopolymers
Xylans
titanium dioxide
arabinoxylan
Durapatite
Titanium